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CARMEN

CARs and multiphoton Microscopy ENabled

carmen

Objective of the project

Within the project CARMEN a versatile multimodal microscopy platform combining the mutual complementary techniques of two-photon excited fluorescence, CARS and STED microscopy for ex vivo biopsies will be developed. The CARMEN imaging system will allow to facilitate intraoperative histology by reducing the unused off-time during surgery and enabling a fast and comprehensive examination of specimens with super-resolution. While the chemical specificity of traditional Raman spectroscopy is excellent, the signal is extremely weak which limits its use as a fast imaging modality. Coherent anti-Stokes Raman scattering is much stronger than the conventional Raman signal and, thereby, enables high-speed, label-free chemical-specific imaging.

The CARMEN project is supported by the Walloon Region in the framework of Eurostars Programme, aimed at R&D performing SMEs.

Contribution of Multitel

Multitel develops a fiber laser system emitting synchronous femtosecond optical pulses (< 100 fs duration) to perform multiphoton imaging and Fourier limited optical pulses (10 ps duration and <0.3 nm spectral width) to perform CARS.

Applications

Imaging et spectroscopy.

Coordinator

Partners

  • Laser Zentrum Hannover - LZH (Germany)
  • Deltatec (Belgium)
  • Laserspec (Belgium)
  • Multitel ( Belgium)

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